Humane pluripotentne NT2/D1 ćelije imobilizovane u alginatnim mikrovlaknima: 3D sistem za testiranje uticaja bioaktivnih jedinjenja Naučni rad
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Učestala konzumacija energetskih pića (engl. energy drinks-ED) i njihova kombinacija sa alkoholom (engl. energy drinks mixed with alcohol-AmED) postala je rastući trend među mladim ljudima. Proklamovano povećanje psihičkih i fizičkih performansi usled konzumiranja ED, koji u svom sastavu sadrže visoke doze stimulativnih sastojka - kofeina i taurina, može dovesti do neželjenih posledica, prvenstveno na funkcije centralnog nervnog sistema (CNS) i kardiovaskularnog sistema. Uprkos ključnoj ulozi u razviću i adultnoj homeostazi, istraživanja o potencijalnom efektu konzumacije ED i AmED na matične i progenitorske ćelije su malobrojne. U radu je predstavljena optimizacija 3D model sistema na bazi alginatnih mikrovlakana za testiranje uticaja bioaktivnih jedinjenja na humane NT2/D1 embrionalne karcinomske ćelije, široko korišćeni model sistem kao pandan humanim matičnim ćelijama. Ispitan je uticaj akutne konzumacije ED i AmED na vijabilnost ovih ćelija i uz pomoć matematičkog modelovanja evaluirana je efikasnost prenosa mase ispitivanih komponenti do imobilisanih ćelija. Dobijeni rezultati pokazuju da ovaj model sistem omogućava optimalan rast i proliferaciju NT2/D1 pluripotentnih ćelija i uniformnu distribuciju ispitivanih komponenti kroz alginatna mikrovlakna. Simulirana akutna konzumacija ED i AmED nije uticala na vijabilnost NT2/D1 ćelija u 3D sistemu, za razliku od 2D modela gde je kofein doveo do malog ali statistički značajnog pada vijabilnosti.
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Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
Grant numbers 451-03-66/2024-03/200042;451-03-65/2024-03/200135;451-03-66/2024-03/200287
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