Validacija metode za analizu etanola u biološkim i nebiološkim uzorcima i njena primena u toksikologiji Stručni rad
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Jednostavna, ekonomična i brza metoda "head-space" gasne hromatografije sa plamenojonizujućim detektorom (engl. headspace gas chromatography coupled with flame ionization detection, HS-GC/FID) za određivanje etanola, razvijena je i validirana radi primene u kliničke i forenzičke toksikološke svrhe.HS-GC/FID se često koristi za određivanje etanola u različitim biološkim i nebiološkim uzorcima. Kalibraciona kriva metode je bila linearna u rasponu od 0,15 do 4,00 g dm-3 (r2 = 0,9999) sa adekvatnom tačnošću (99,12–105,77 %) i preciznošću. Granica detekcije (engl. limit of detection, LOD) bila je 0,006 g dm-3. Metoda je bila kvantitativna (engl. limit of quantification, LOQ) iznad koncentracije etanola od 0,02 g dm-3. Nova metoda je uspešno korišćena za određivanje etanola u biološkim uzorcima pacijenata, učesnika saobraćajnih nezgoda, izvršioca krivičnih dela, postmortem uzorcima, nebiološkim uzorcima poput alkoholnih pića, biljnih preparata na bazi alkohola, kozmetičkih preparata, itd. Metoda je jednostavna za izvođenje, što je čini pogodnom ne samo za svakodnevnu praksu kliničko-biohemijskim i forenzičkim laboratorijama, već i u različitim poljima industrije (farmaceutski preparati, dijetetski suplementi, kozmetički preparati...). Takođe, prikazana je i primena ove metode za određivanje sadržaja etanola u različitim uzorcima povezanih sa kliničko-forenzičkim slučajevima.
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