Optimization of bioethanol production from soybean molasses using different strains of Saccharomyces cerevisiae

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Zorana Rončević
Bojana Bajić
Siniša Dodić
Jovana Grahovac
Radmila Pajović-Šćepanović
Jelena Dodić


Bioethanol technology represents an important scientific research area because of the high market value and wide availability of its primary and by-products. Worldwide interest in utilizing bioethanol as a renewable and sustainable energy source has significantly increased in the last few years due to limited reserves of fossil fuels and concerns about climate change. Therefore, improvement of the bioethanol production process is a priority research field at the international scale, due to both economic and environmental reasons. The aim of this study was to optimize production of bioethanol from soybean molasses based media using response surface methodology. Three different strains of the yeast Saccharomices cerevisiae, commercially available in dried form, were used as production microorganisms, and the best results were obtained by using dried baker’s yeast. The results of optimization of alcoholic fermentation using dried baker’s yeast indicate that the highest value of the overall desirability function (0.945) is obtained when the initial sugar content is 18.10 % (w/v) at the fermentation time of 48.00 h. At these conditions the model predicts that bioethanol concentration is 8.40 % (v/v), yeast cell number 2.21·108 cells/mL and the residual sugar content is 0.35 % (w/v).

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Biochemical Engineering - General


Dodić S, Popov S, Dodić J, Ranković J, Zavargo Z, JevtićMučibabić R.Bioethanol production from thick juice as intermediate of sugar beet processing. Biomass Bioenergy. 2009; 33: 822-827.

Zabed H, Sahu JN, Suely A, Boyce AN, Faruq G. Bioethanol production from renewable sources: Current perspectives and technological progress. Renew Sust Energ Rev. 2017; 71: 475–501.

Ivančić Šantek M, Miškulin E, Beluhan S, Šantek B. Novi trendovi u proizvodnji etanola kao biogoriva. Kem. Ind. 2016; 65: 25−38 (in Croatian).

MojovićLj, Pejin D, Rakin M, Pejin J, Nikolić S, Djukić-Vuković A. How to improve the economy of bioethanol production in Serbia. Renew Sust Energ Rev. 2012; 16: 6040-6047.

Predojević ZJ. Postupci pripreme lignocelulozne sirovine za dobijanje bioetanola. Hem. Ind. 2010; 64: 283-293 (in Serbian).

Grahovac J, Dodić J, Jokić A, Dodić S, Popov S. Optimization of ethanol production from thick juice: A response surface methodology approach. Fuel. 2012; 93: 221-228.

Siqueira PF, Karp SG, Carvalho JC, Sturm W, Rodríguez-León JA, Tholozan JL, Singhania RR, Pandey A, Soccol CR. Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae at laboratory, pilot and industrial scales. Bioresour Technol. 2008; 99: 8156–8163.

Romao B, da Silva FB, de Resende MM, Cardoso VL. Ethanol Production from Hydrolyzed Soybean Molasses. Energ. Fuel. 2012; 26: 2310-2316.

ŽivanovićLj, Popović V. Proizvodnja soje (Glycine max.) u svetu i kod nas. U Zborniku radova XXI SAVETOVANJE O BIOTEHNOLOGIJI. 2016; 21: 129-135.

Caldeirão L, Tanaka C, Ida E, Spinosa W. Modeling and kinetic study of bio-ethanol production from soy protein concentrate by-product. Food Sci. Technol. 2016; 36: 369-374.

Zabed H, Faruq G, Sahu JN, Azirun MS, Hashim R, Boyce AN. Bioethanol Production from Fermentable Sugar Juice. The Scientific World Journal. 2014; Article ID 957102: 1-11.

Kalil SJ, Maugeri F, Rodrigues MI. Response surface analysis and simulation as a tool for bioprocess design and optimization. Process Biochem. 2000; 35: 539-550.

Madamba PS. The Response Surface Methodology: An Application to Optimize Dehydration Operations of Selected Agricultural Crops. LWT. 2002; 35: 584–592.

Popov S, Ranković J, Dodić J, Dodić S, Jokić A. Bioethanol Production from Raw Juice as Intermediate of Sugar Beet Processing: A Response Surface Methodology Approach. Food Technol. Biotechnol. 2010; 48: 376-383.

Grahovac JA, Dodić JM, Dodić SN, Popov SD, Jokić AI, Zavargo ZZ. Optimization of bioethanol production from intermediates of sugar beet processing by response surface methodology. Biomass Bioenergy. 2011; 35: 4290-4296.

Betiku E, Taiwo AE. Modeling and optimization of bioethanol production from breadfruit starch hydrolyzatevis-a-vis response surface methodology and artificial neural network. Renew.Energ. 2015; 74: 87-94.

Rončević Z, Dodić J, Grahovac J, Dodić S, Bajić B, Vučurović D, Tadijan I. Definition of optimum basic nutrients ratio in media for bioethanol production with immobilised yeast cells. International Journal of Innovation and Sustainable Development. 2017; 11: 53-68.

Sewsynker-Sukai Y, Kana EBG. Simultaneous saccharification and bioethanol production from corn cobs: Process optimization and kinetic studies. Bioresour. Technol. 2018; 262: 32-41.

Tahir A, Aftab M, Farasat T. Effect of cultural conditions on ethanol production by locally isolated Saccharomyces cerevisiae Bio-07. J. App. Pharm. 2010; 3: 72-78.

Amadi PU, Ifeanacho MO. Impact of changes in fermentation time, volume of yeast, and mass of plantain pseudo-stem substrate on the simultaneous saccharification and fermentation potentials of African land snail digestive juice and yeast. J. Genet. Eng. Biotechnol. 2016; 14: 289-297.

Laluce C, Tognolli JO, de Oliveira KF, Souza CS, Morais MR. Optimization of temperature, sugar concentration, and inoculum size to maximize ethanol production without significant decrease in yeast cell viability. Appl. Microbiol. Biotechnol. 2009; 83: 627-637.

Phukoetphim N, Salakkam A, Laopaiboon P, Laopaiboon L. Improvement of ethanol production from sweet sorghum juice under batch and fed-batch fermentations: Effects of sugar levels, nitrogen supplementation, and feeding regimes. Electron. J. Biotechn. 2017; 26: 84-92.

Bai FW, Anderson WA, Moo-Young M. Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol. Adv. 2008; 26: 89-105.

Dodić JM, Rončević ZZ, Grahovac JA, Bajić BŽ, Korolija OS. BiosintezakomponentiantifungalnogdelovanjapremaAspergillus spp. primenomStreptomyces hygroscopicus. Hem. Ind. 2015; 69: 201-208 (in Serbian).

Grahovac J, Dodić J, Rončević Z, Dodić S, Vučurović D. Distillate composition of fermented media based on by-products of sugar beet processing. Rom. Biotech. Lett. 2017; DOI: 10.26327/RBL2017.79.

Pătraşcu E, Râpeanu G, Bonciu C, Vicol C, Bahrim G. Investigation of yeast performances in the fermentation of beet and cane molasses to ethanol production. Ovidius Univ. Annals Chem. 2009; 20: 199-204.

Azhar SHM, Abdulla R, Jambo SA, Marbawi H, Gansau JA, Faik AAM, Rodrigues KF. Yeasts in sustainable bioethanol production: A review. Biochem. Biophys. Rep. 2017; 10: 52-61.

Zheng D, Zhang K, Gao K, Liu Z, Zhang X, Li O, Sun J, Zhang X, Du F, Sun P, Qu A, Wu X. Construction of Novel Saccharomyces cerevisiae Strains for Bioethanol Active Dry Yeast (ADY) Production. PLoS ONE 2013; 8: 1-12.

Ranković J, Dodić J, Dodić S, Popov S. Bioethanol production from intermediate products of sugar beet processing with different types of Saccharomyces cerevisiae. Chem. Ind. Chem. Eng. Q. 2009; 15: 13-16.

Karp SG, Woiciechowski AL, Letti LAJ, Soccol CR. Bioethanol from Soybean Molasses. In: Soccol CR, Brar SK, Faulds C, Ramos LP, eds. Green Fuels Technology. Springer International Publishing Switzerland. 2016: 241-254.

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