Niskoenergetske nanoemulzije kao nosači za etarska ulja u topikalnim formulacijama za antioksidantnu zaštitu kože Naučni rad
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U ovoj studiji nekoliko različitih etarskih ulja (EU): bosiljak – BO, matičnjak – MA i origano – OR inkorporirano je u nanoemulzije (NE) kao potencijalne nosače za prirodne i osetljive bioaktivne sastojke. NE su pripremljene pomoću metode inverzije faza (engl. phase inversion composition – PIC method) koja predstavlja niskoenergetski i ekonomičan postupak izrade. Fizičkohemijska stabilnost nanoemulzija potvrđena je analizom raspodele veličina kapi, merenjem električne provodljivosti i pH vrednosti, kao i optičkom mikroskopijom. Nađeno je da su vrsta EU i koncentracija smeše surfaktanata i uljane faze dva ključna faktora koja su uticala na karakteristike i stabilnost dobijenih emulzija. Tehnikom Ramanske spektroskopije potvrđeni su glavni aktivni sastojci etarskih ulja i detektovane su moguće interakcije sa nanonosačem, što predstavlja noviju primenu pomenute tehnike. Pokazano je da antioksidantna aktivnost prema DPPH radikalu u metanolu zavisi od koncentracije, sa sličnim trendom za čista etarska ulja i za nanoemulzije sa uljima (OR> MA> BO). Međutim, ABTS test u vodenoj sredini pokazao je izrazite promene u redosledu aktivnosti sa povećanjem koncentracije EU i nakon nanonizacije EU. Generalno, dokazano je da je OR-NE najefikasniji i najstabiliniji sistem, s obzirom da je ulje origana ispoljilo ulogu kostabilizatora u formulaciji, uz istovremeno očuvanje njegove veoma visoke antioksidantne aktivnosti u obliku nanoemulzije, tokom 6 meseci čuvanja.
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