Aqueous extract of strawberry (Fragaria x ananassa Duch.) leaves as a stabilizing agent in the synthesis of bio-active silver nanoparticles

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Marija Stevanović
Ljiljana Stanojević
Bojana Danilović
Sanja Stojanović
Stevo Najman
Milorad Cakić
Dragan Cvetković


The aim of the presented work was to investigate the potential of aqueous extract of cultivated strawberry (Fragaria x ananassa Duch.) leaves for stabilization of silver nanoparticles (AgNPs-E) synthesized at room (RT) and boiling temperature (BT). The synthesis and stability of AgNPs-E were monitored by UV-Vis spectroscopy confirming high stability of the AgNPs-E in the dark at room temperature. The Fourier-transform infrared spectra suggest that molecules containing oxygen and nitrogen functional groups (NH, (NH)C=O, CNO, C-O-C and OH) participate in the reduction and stabilization of formed nanoparticles. As determined by the DPPH test, AgNPs-E synthesized at RT exerted higher antioxidant activity as compared to AgNPs-E synthesized at BT (EC50 values of 0.025 and 0.039 mg cm-3, respectively). Also, the AgNPs-E synthesized at RT exerted higher antibacterial activity against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus subtilis and Bacillus luteus. Examination of the AgNPs-E on HeLa and MDCK cell lines showed concentration-dependent and cell line specific effects on the cell viability as evaluated by the MTT test. The obtained results indicate that synthesized AgNPs-E can be used as a base material in production of pharmaceutical preparations for potential skin applications.


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Stevanović, M., Stanojević, L., Danilović, B., Stojanović, S., Najman, S., Cakić, M., & Cvetković, D. (2021). Aqueous extract of strawberry (Fragaria x ananassa Duch.) leaves as a stabilizing agent in the synthesis of bio-active silver nanoparticles. HEMIJSKA INDUSTRIJA (Chemical Industry), 74(6), 365–376.
Biochemical Engineering - General


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