Main Article Content
In this study, immobilization of laccase from Trametes versicolor on eight Lifetech™supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech™ ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech™ ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset® Violet B, Lanaset® Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:
- the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
- the right to produce prepublications, reprints, and special editions of the Manuscript;
- the right to translate the Manuscript into other languages;
- the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
- the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers;
- the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
- the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.
Kunamneni A, Plou F, Ballesteros A, Alcalde M. Laccases and Their Applications: A Patent Review. Recent Pat Biotechnol. 2008;2(1):10-24.
Claus H. Laccases: Structure, reactions, distribution. Micron. 2004;35(1-2):93-96.
De La Torre M, Martín-Sampedro R, Fillat Ú, Eugenio ME, Blánquez A, Hernández M, Arias ME, Ibarra D. Comparison of the efficiency of bacterial and fungal laccases in delignification and detoxification of steam-pretreated lignocellulosic biomass for bioethanol production. J Ind Microbiol Biotechnol. 2017;44(11):1561-1573.
Ilyas S, Rehman A. Decolorization and detoxification of synozol red HF-6BN azo dye, by Aspergillus niger and Nigrospora sp. Iran J Environ Heal Sci Eng. 2013;10(12).
Viswanath B, Rajesh B, Janardhan A, Kumar AP, Narasimha G. Fungal laccases and their applications in bioremediation. Enzyme Res. 2014;2014.
Legerská B, Chmelová D, Ondrejovič M. Decolourization and detoxification of monoazo dyes by laccase from the white-rot fungus Trametes versicolor. J Biotechnol. 2018;285:84-90.
Przystaś W, Zabłocka-Godlewska E, Grabińska-Sota E. Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports. Brazilian J Microbiol. 2018;49(2):285-295.
Sheldon RA. Enzyme immobilization: The quest for optimum performance. Adv Synth Catal. 2007;349(8-9):1289-1307.
Minussi RC, Pastore GM, Durán N. Potential applications of laccase in the food industry. Trends Food Sci Technol. 2002;13(6-7):205-216.
Mousty C, Vieille L, Cosnier S. Laccase immobilization in redox active layered double hydroxides: A reagentless amperometric biosensor. Biosens Bioelectron. 2007;22(8):1733-1738.
Durán N, Rosa MA, D’Annibale A, Gianfreda L. Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: A review. Enzyme Microb Technol. 2002;31(7):907-931.
Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol. 2007;40(6):1451-1463.
Fernández-Fernández M, Sanromán MÁ, Moldes D. Recent developments and applications of immobilized laccase. Biotechnol Adv. 2013;31(8):1808-1825.
Bebić J, Banjanac K, Ćorović M, Milivojević A, Simović M, Marinković A, Bezbradica D. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation. Chinese J Chem Eng. 2020;in press.
Bebić J, Banjanac K, Rusmirović J, Ćorović M, Milivojević A , Simović M, Marinković A, Bezbradica D. Amino-modified kraft lignin microspheres as a support for enzyme immobilization. RSC Adv. 2020;10:21495-21508.
Mazlan SZ, Hanifah SA. Effects of Temperature and pH on Immobilized Laccase Activity in Conjugated Methacrylate-Acrylate Microspheres. Int J Polym Sci. 2017;2017.
Jiang DS, Long SY, Huang J, Xiao HY, Zhou JY. Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres. Biochem Eng J. 2005;25(1):15-23.
Radojković-Veličković M, Mijin D. Organske Boje i Pigmenti.; 2001.
Maamar M, Naimi I, Mkadem Y, Souissi N, Bellakhal N. Electrochemical oxidation of Bromothymol blue: Application to textile industrial wastewater treatment. J Adv Oxid Technol. 2015;18(1):105-113.
Chijioke Elijah O, Nonso Collins O, Callistus Obumneme O, Jessica N-B. Application of Modified Agricultural Waste in the Adsorption of Bromocresol Green Dye. Asian J Chem Sci. 2020:15-24.
Purolite. Purolite LifetechTM ECR Enzyme Immobilization Resins. 2014:13.
Ride JP. The effect of induced lignification on the resistance of wheat cell walls to fungal degradation. Physiol Plant Pathol. 1980;16(2).
Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
Zhang DH, Yuwen LX, Peng LJ. Parameters affecting the performance of immobilized enzyme. J Chem. 2013.
Mohamad NR, Marzuki NHC, Buang NA, Huyop F, Wahab RA. An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. Biotechnol Biotechnol Equip. 2015;29(2):205-220.
Wang A, Wang H, Zhu S, Zhou C, Du Z, Shen S. An efficient immobilizing technique of penicillin acylase with combining mesocellular silica foams support and p-benzoquinone cross linker. Bioprocess Biosyst Eng. 2008;31(5):509-517.
Reis CLB, de Sousa EYA, de França Serpa J, Oliveira RC, Dos Santos JCS. Design of immobilized enzyme biocatalysts: Drawbacks and opportunities. Quim Nova. 2019;42(7):768-783.
Dean Brady · Justin Jordaan. Microbial and Enzyme Technology. In: Advances in Enzyme Immobilisation. ; 2009:1-36.
Piontek K, Antorini M, Choinowski T. Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-Å resolution containing a full complement of coppers. J Biol Chem. 2002;277(40):37663-37669.
P. Baldrian. Fungal laccases - occurrenceand properties. FEMS Microbiol Rev. 2006;30:215-242.
Gascón V, Díaz I, Márquez-Álvarez C, Blanco RM. Mesoporous silicas with tunable morphology for the immobilization of laccase. Molecules. 2014;19(6):7057-7071.
Yinghui D, Qiuling W, Shiyu F. Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier. Lett Appl Microbiol. 2002;35(6):451-456.
Brown D, Hitz HR, Schäfer L. The assessment of the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria experience with a screening test. Chemosphere. 1981;10(3):245-261.
Ramírez-Montoya LA, Hernández-Montoya V, Montes-Morán MA, Jáuregui-Rincón J, Cervantes FJ. Decolorization of dyes with different molecular properties using free and immobilized laccases from Trametes versicolor. J Mol Liq. 2015;212:30-37.
Diorio LA, Mercuri AA, Nahabedian DE, Forchiassin F. Development of a bioreactor system for the decolorization of dyes by Coriolus versicolor f. antarcticus. Chemosphere. 2008;72(2):150-156.
Chao C, Guan H, Zhang J, Liu Y, Zhao Y, Zhang B. Immobilization of laccase onto porous polyvinyl alcohol/halloysite hybrid beads for dye removal. Water Sci Technol. 2018;77(3):809-818.