Potential application as a bio-coagulant for Moringa oleifera seeds
Original scientific paper
DOI:
https://doi.org/10.2298/CICEQ240313008ZKeywords:
Moringa oleifera seeds, n-hexane, bio-coagulant, water treatmentAbstract
This study focused on producing a natural bio-coagulant from moringa seeds. Moringa seed extract is a natural coagulant for the treatment of water containing suspended solids and colloids and is obtained through grinding, oil extraction, protein extraction from the solids using 2 M NaCl solution, protein separation by centrifugation followed by filtration. This study investigated the production and use of bio-coagulants. Parameters investigated when producing the bio-coagulant include the effects of temperature on the removal of moisture content in the moringa seeds, the type of solvent on the oil yield, and varying the volume of n-hexane for oil extraction. Parameters investigated when using the bio-coagulant include the effect of dosage of bio-coagulant on pH and turbidity removal efficiency, and the performance of the bio-coagulant at low, medium, and high turbidity levels. The optimum temperature and time obtained by drying the moringa seeds was at 100 °C for 30 min and the highest oil yield obtained was 31% when using n-hexane as the solvent. Turbidity removal efficiency went up to 96.4 % when using 50 mg/L of the bio-coagulant at a pH of 6.1. High turbidity removal was achieved at low bio-coagulant dosages which was regarded as a breakthrough finding for this research.
References
[1] A. Benettayeb, M. Usman, C. C. Tinashe, T. Adam, and B. Haddou, Environ. Sci. Pollut. Res., vol. 29, no. 32, pp. 48185–48209, 2022, doi: 10.1007/s11356-022-19938-w.
[2] A. Ndabigengesere, K. S. Narasiah, and B. G. Talbot, Water Res., vol. 29, no. 2, pp. 703–710, 1995, doi: 10.1016/0043-1354(94)00161-Y.
[3] S. Nouhi, H. M. Kwaambwa, P. Gutfreund, and A. R. Rennie, Sci. Rep., vol. 9, no. 1, pp. 1–10, 2019, doi: 10.1038/s41598-019-54069-2.
[4] C. S. T. Araújo et al., Water Sci. Technol., vol. 62, no. 9, pp. 2198–2203, 2010, doi: 10.2166/wst.2010.419.
[5] L. L. Salazar Gámez, M. Luna-del Risco, and R. E. S. Cano, Environ. Monit. Assess., vol. 187, no. 10, 2015, doi: 10.1007/s10661-015-4793-y.
[6] S. Boulaadjoul, H. Zemmouri, Z. Bendjama, and N. Drouiche, Chemosphere, vol. 206, pp. 142–149, 2018, doi: 10.1016/j.chemosphere.2018.04.123.
[7] W. L. Ang and A. W. Mohammad, J. Clean. Prod., vol. 262, p. 121267, 2020, doi: 10.1016/j.jclepro.2020.121267.
[8] N. Ueda Yamaguchi et al., Process Saf. Environ. Prot., vol. 147, no. April, pp. 405–420, 2021, doi: 10.1016/j.psep.2020.09.044.
[9] D. L. Villaseñor-Basulto, P. D. Astudillo-Sánchez, J. del Real-Olvera, and E. R. Bandala, J. Water Process Eng., vol. 23, no. February, pp. 151–164, 2018, doi:10.1016/j.jwpe.2018.03.017.
[10] L. Liang, C. Wang, S. Li, X. Chu, and K. Sun, Food Sci. Nutr., vol. 7, no. 5, pp. 1754–1760, 2019, doi: 10.1002/fsn3.1015.
[11] H. Barakat and G. A. Ghazal, Food Nutr. Sci., vol. 07, no. 06, pp. 472–484, 2016, doi: 10.4236/fns.2016.76049.
[12] M. Alain Mune Mune, E. C. Nyobe, C. Bakwo Bassogog, and S. R. Minka, Cogent Food Agric., vol. 2, no. 1, 2016, doi: 10.1080/23311932.2016.1213618.
[13] K. Bombo, T. Lekgoba, O. Azeez, and E. Muzenda, Environ. Clim. Technol., vol. 25, no. 1, pp. 151–160, 2021, doi: 10.2478/rtuect-2021-0010.
[14] T. A. Aderinola, T. N. Fagbemi, V. N. Enujiugha, A. M. Alashi, and R. E. Aluko, Heliyon, vol. 4, no. 10, p. e00877, 2018, doi: 10.1016/j.heliyon.2018.e00877.
[15] V. Javed, S. Javed, H. U. Saeed, T. Abbas, and I. Technology, DSSR, vol. 3, no. 1, pp. 77–88, 2025.
[16] M. M. Maroneze, L. Q. Zepka, J. G. Vieira, M. I. Queiroz, and E. Jacob-Lopes, A&A, vol. 9, no. 3, pp. 445–458, 2014, doi: 10.4136/1980-993X.
[17] J. R. Silva and D. S. Oliveira, Clean Technol., vol. 6, no. 2, pp. 625–645, 2024, doi: 10.3390/cleantechnol6020033.
[18] R. W. Saa, E. N. Fombang, E. B. Ndjantou, and N. Y. Njintang, Food Sci. Nutr., vol. 7, no. 6, pp. 1911–1919, 2019, doi: 10.1002/fsn3.1057.
[19] N. E. Nwaiwu and A. A. Bello, Res. J. Appl. Sci. Eng. Technol., vol. 3, no. 6, pp. 505–512, 2011.
[20] R. S. Putra, M. Ayu, and R. Y. Amri, Key Eng. Mater., vol. 840 KEM, no. May, pp. 29–34, 2020, doi: 10.4028/www.scientific.net/kem.840.29.
[21] A. B. A., IOSR J. Agric. Vet. Sci., vol. 1, no. 5, pp. 12–21, 2012, doi: 10.9790/2380-0151221.
[22] E. Ajav and O. Fakayode, Agrosearch, vol. 13, no. 1, p. 115, 2013, doi: 10.4314/agrosh.v13i1.11.
[23] D. O. Oloyede, N. A. Aviara, and S. K. Shittu, J. Biosyst. Eng., vol. 40, no. 3, pp. 201–211, 2015, doi: 10.5307/jbe.2015.40.3.201.
[24] M. M. S. Arreola, J. R. L. Canepa, and J. R. H. Barajas, Interciencia, vol. 41, no. 8, pp. 548–551, 2016.
[25] Q. Du, Y. Wu, S. Xue, and Z. Fu, LWT, vol. 155, p. 112988, 2022, doi: 10.1016/j.lwt.2021.112988.
[26] G. S. Madrona, I. G. Branco, V. J. Seolin, and B. De, no. May, 2012, Acta Sci. Technol, doi: 10.4025/actascitechnol.v34i3.9605.
[27] A. Gautier, C. M. Duarte, and I. Sousa, “Moringa oleifera Seeds Characterization and Potential Uses as Food,” Foods, vol. 11, no. 11, 2022, doi: 10.3390/foods11111629.
[28] A. Jain and R. S. B. M. C. Radha, J. Food Sci. Technol., vol. 56, no. 4, pp. 2093–2104, 2019, doi: 10.1007/s13197-019-03690-0.
[29] B. O. Mbah, P. E. Eme, and O. F. Ogbusu, Pakistan J. Nutr., vol. 11, no. 3, pp. 211–215, 2012, doi: 10.3923/pjn.2012.211.215.
[30] O. a Abiodun, J. a Adegbite, and O. Omolola, Glob. J. Sci. Front. Res., vol. 12, no. 5, pp. 13–17, 2012.
[31] P. Bridgemohan, R. Bridgemohan, and M. Mohamed, African J. Food Sci. Technol., vol. 5, no. 5, pp. 125–128, 2014.
[32] E. Kusumawati, Keryanti, E. M. Widyanti, F. Waluya, and Risnawati, vol. 198, no. Issat, pp. 365–370, 2020, doi: 10.2991/aer.k.201221.060.
[33] M. H. Ng and M. S. Elshikh, Ind. Domest. Waste Manag., vol. 1, no. 1, pp. 1–11, 2021, doi: 10.53623/idwm.v1i1.41.
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