Iskorišćenje nus-proizvoda agro-industrije za proizvodnju dekstransaharaze pomoću bakterije Leuconostoc mesenteroides T3: optimizacija procesa metodom odzivnih površina Naučni rad
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Apstrakt
Dekstransaharaza (DS) je glukoziltransferaza (E. C. 2.4.1.5.) koja katalizuje prenos ostataka glukoze iz saharoze u polimer dekstrana, pri čemu se oslobađa fruktoza. Ovaj enzim je povezan sa širokim spektrom primene dekstrana i oligosaharida. Proizvodnja dekstransaharaze pomoću bakterije Leuconostoc mesenteroides T3 optimizovana je metodom odzivnih površina korišćenjem centralnog kompozitnog dizajna. Za optimizaciju su izabrane tri promenljive: koncentracija džibre, koncentracija saharoze i koncentracija jona mangana. Rezultati su pokazali da koncentracije saharoze i jona mangana imaju pozitivan linearni efekat na proizvodnju DS dok su sve interakcije (džibra-Mn2+, džibra-saharoza i saharoza-Mn2+) imale značajan uticaj na proizvodnju DS. Na osnovu eksperimentalnih podataka i numeričke optimizacije, dobijen je maksimalni prinos DS od 3.391 ± 0.131 U cm-3 u podlozi sa 64.33 % džibre, 5.30 % saharoze i 0.022 % jona mangana. Naše istraživanje otkrilo je da se džibra u kombinaciji sa melasom šećerne repe kao i saharozom i dodatkom jona mangana može koristiti kao dragocena hranjiva komponenta za rast bakterija mlečne kiseline i proizvodnju DS. Takođe, uzimajući u obzir poreklo supstrata, upotreba industrijskih nusproizvoda na ovaj način ima veliku ekološku važnost.
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