Improving the stability of a probiotic product with Lactiplantibacillus plantarum 299v by introducing flow pack bags Technical paper
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Abstract
Probiotic products are becoming more common in everyday use around the world, while at the same time, the interest of scientists in researching probiotic production and use is increasing. Stability of a probiotic product in pharmaceutical production is affected by the choice of probiotic strain, formulation, and packaging. Packaging is the final stage of production and presents a crucial factor for the stability of probiotic products to maintain declared probiotic viability during the products' shelf life. The present research describes the influence of additional packaging material on the encapsulated probiotic product, which contains Lactiplantibacillus plantarum 299v. In specific, the effect of additional blister protection within flow pack bags was investigated. Blisters were made of a chloride/polyvinylidene chloride/polyethylene-triplex foil (PVC/PVdC/PE foil) and aluminum foil. Viability of probiotic lactobacilli cells protected in blisters only was compared to those packed in flow pack bags filled with nitrogen as an inert gas. Better protection of probiotic cells from oxygen, light, and moisture was determined in the capsules in the latter case. In specific, introduction of additional blister protection in flow pack bags resulted in ~11 % higher probiotic viability when compared to the other blister samples without such protection after 24 months, and therefore it enabled more efficient storage of the product during use.
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References
WGO Review Team. World Gastroenterology Organisation Global Guidelines- Probiotics and prebiotics. World Gastroenterology Organisation; 2017 https://www.worldgastroenterology.org/UserFiles/file/guidelines/probiotics-and-prebiotics-english-2017.pdf
FAO (Food and Agriculture Organization of the United Nations), WHO (World Health Organization). Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Cordoba, Argentina; 2001. https://www.iqb.es/digestivo/pdfs/probioticos.pdf.
Salminen S, Ouwehand AC, Isolauri E. Clinical Applications of Probiotic Bacteria. Int Dairy J. 1998; 8(5-6): 563-572 https://doi.org/10.1016/S0958-6946(98)00077-6.
Zheng J, Wittouck S, Salvetti E, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int J Syst Evol Microbiol. 2020; 70 (4): 2782-2858 https://doi.org/10.1099/ijsem.0.004107.
Korčok D, Tršić-Milanović N, Ivanović N, Đorđević B. Development of Probiotic Formulation for the Treatment of Iron Deficiency Anemia. Chem Pharm Bull. 2018; 66(4): 347–352 https://doi.org/10.1248/cpb.c17-00634.
Arvidsson Nordstrom E, Teixeira C, Montelius C, Jeppsson B, Larsson N. Lactiplantibacillus plantarum 299v (LP299V®): three decades of research. Benef Microbes. 2021; 12(5): 441-465 https://doi.org/10.3920/bm2020.0191.
Abdelazez A, Abdelmotaal H, Zong-Tao Z, Fang-Fang J, Sami R, Zhang L, Rahman Al Twaha A, Meng X. Potential benefits of Lactiplantibacillus plantarum as probiotic and its advantages in human health and industrial applications. Adv Environ Biol. 2018; 12(1): 16-27 http://dx.doi.org/10.22587/aeb.2018.12.1.4.
Axling U, Önning G, Martinsson Niskanen T, Larsson N, Hansson SR, Hulthén L. The effect of Lactiplantibacillus plantarum 299v together with low dose of iron on iron status in healthy pregnant women: A randomized clinical trial. Acta Obstet Gynecol Scand. 2021; 100(9): 1602–1610 https://doi.org/10.1111%2Faogs.14153.
Ipek G, Juneja V, Ahmedna M. Probiotics in food safety and human health. 1st ed., Boca Raton, FL, USA: Taylor and Francis Group; 2006 https://doi.org/10.1201/9781420027570.
Korčok D, Tršić- Milanović N, Ilić M, Mitić B, Đorđević B, Ivanović N. Improving the vability and stability of probiotic product with Saccharomyces boulardii DBVPG. Hem Ind. 2021; 75(1): 25-30 https://doi.org/10.2298/HEMIND201211008K.
Wang G, Chen Y, Xia Y, Song X, Ai L. Characteristics of Probiotic Preparations and Their Applications. Foods. 2022; 11: 2472 https://doi.org/10.3390/foods11162472.
Fenster K, Freeburg B, Holland C, Wong C, Rønhave Laursen R, Ouweghand AC. The Production and Delivery of Probiotics: A Review of a Practical Approach. Microorganisms. 2019; 7(3): 83 https://doi.org/10.3390/microorganisms7030083.
Saarela M. Probiotic technology Maintaining viability and stability. Agro Food Industry Hi Tech. 2007; 18 (4): 19-21 https://www.researchgate.net/publication/289771526_Probiotic_technology_Maintaining_viability_and_stability.
Uddin M, Mamun A, Rashid M, Asaduzzaaman M. In-process and Finished Products Quality Control Tests for Pharmaceutical Capsules According to Pharmacopoeias. Int. J Pharm Res. 2015; 9(2): 1-9 https://doi.org/10.9734/bjpr%2F2016%2F22044.
Dao H, Lakhani P, Police A, Kallakunta V, Ajjarapu SS, Wu KW, Ponkshe P, Repka MA, Murthy SN. Microbial Stability of Pharmaceutical and Cosmetic Products. AAPS Pharm SciTech. 2018; 19(1): 60–78 https://doi.org/10.1208/s12249-017-0875-1.
Das S, Bhattacharjee D, Manna A, Basu S, Chowdhury S, Mukherjee S, Bhattacharyya BK. Effect of Different Excipients and packaging Materials on Commercial Preparation of Probiotic Formulation. Int J Pharm Sci Res. 2014; 5(5): 1830-1836 http://dx.doi.org/10.13040/IJPSR.0975-8232.5(5).1830-36.
Kolaček S, Hojsak I, Berni Canani R, Guarino A, Indrio F, Orel R, Pot B, Shamir R, Szajewska H, Vandenplas Y, van Goudoever J, Weizman Z, and ESPGHAN Working Group for Probiotics and Prebiotics. Commercial Probiotic Products: A Call for Improved Quality Control. A Position Paper by the ESPGHAN Working Group for Probiotics and Prebiotics. J Pediatr Gastroenterol Nutr. 2017; 65(1): 117–124 https://doi.org/10.1097/mpg.0000000000001603.
De Man JC, Rogosa M, Elisabeth Sharpe M. A medium for the cultivation of lactobacilli. J Appl Microbiol. 1960; 23: 130-135 https://doi.org/10.1111/j.1365-2672.1960.tb00188.x.
Tranberg A, Klarin B, Johnson J, Pahlman LI. Efficacy of Lactiplantibacillus plantarum 299 and 299 v against nosocomial oropharyngeal pathogens in vitro and as an oral prophylactic treatment in a randomized, controlled clinical trial. Microbiologyopen. 2021; 10(1): 1151 https://doi.org/10.1002/mbo3.1151.
Sánchez Rodríguez M, Castán Urbano H, Muñoz de los Ríos D. Evaluation of Viability of Lactobacillus fermentum CECT 5716 in Gelatin and Gastroresistant Capsules. J Pharm Pharmacol. 2016; 4(8): 413-418 http://dx.doi.org/10.17265/2328-2150/2016.08.009.