The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

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Sandra S. Konstantinović
Milica Z. Zlatković
Jovan T. Ćirić
Slavica B. Ilić
Gordana D. Gojgić Cvijović
Vlada B. Veljković


In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/VIS, 1H NMR and X-ray spectroscopic methods. Yields of 93-98% of the compounds in crude glycerol were achieved within 10-25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (Hexaene H-85 and Azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of Hexaene H-85 and Azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 g/cm3 and 69 µg/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde-phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics. 


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Konstantinović, S. S., Zlatković, M. Z., Ćirić, J. T., Ilić, S. B., Gojgić Cvijović, G. D., & Veljković, V. B. (2018). The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7. HEMIJSKA INDUSTRIJA (Chemical Industry), 71(6), 487–494.
Applied Chemistry


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