Bacillus based microbial formulations: Optimization of the production process

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Sandra Stamenković Stojanović
Ivana Karabegović
Vladimir Beškoski
Nada Nikolić
Miodrag Lazić


Bacillus sp.-based microbial formulations have found wide application in many fields: from pharmacy and medicine to environmental protection and agriculture due to the ability of this species to produce various metabolites and to form endospores. Recently, these products have gained popularity as biopesticidal and phytostimulatory agents, which are a "green" alternative to overused agrochemicals. In order to obtain a high-quality and long-lasting product with desired characteristics, it is necessary to optimize the production process at each stage, which implies coordinating the microbial species, the type and the conditions of microbial cultivation along with formulation technologies. This paper provides a concise overview of the most important findings in this area, regarding characteristics of microbial formulations and specific criteria that need to be met when such a product is formulated. It should serve as a beginning point for everyone starting new research, not just in the field of biofertilization and biological control of plant diseases, but generally in the field of biochemical engineering.


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Stamenković Stojanović, S., Karabegović, I., Beškoski, V., Nikolić, N., & Lazić, M. (2019). Bacillus based microbial formulations: Optimization of the production process. HEMIJSKA INDUSTRIJA (Chemical Industry), 73(3), 169–182.
Biochemical Engineering - General
Author Biographies

Sandra Stamenković Stojanović, University of Niš, Faculty of Technology, Bulevar Oslobođenja 124, 16000 Leskovac

Department for food technology and Biotechnology

PhD student

Teaching assistant 

Ivana Karabegović, University of Niš, Faculty of Technology, Bulevar Oslobođenja 124, 16000 Leskovac

Department for Food technology and Biotechnology 

 Assistant professor 

Vladimir Beškoski, University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade

Department for biochemistry

Nada Nikolić, University of Niš, Faculty of Technology, Bulevar Oslobođenja 124, 16000 Leskovac

Department for Food technology and Biotechnology 

 Full professor 

Miodrag Lazić, University of Niš, Faculty of Technology, Bulevar Oslobođenja 124, 16000 Leskovac

Department for Food technology and Biotechnology 

 Full professor 


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