Uticaj strukture ceftazidima na dobijanje biološki aktivnog celuloznog zavoja
Main Article Content
Abstract
Biološki aktivna vlakna kao nosači ljekovitih preparata imaju poboljšane karakteristike u odnosu na konvencionalne medicinske terapije. Njihove osobine zavise od vrste nosača i od strukture samog lijeka. U ovom radu selektivnom oksidacijom celuloznog zavoja dobijena je oksidovana celuloza (OC) sa različitim sadržajem karboksilnih grupa koja je iskorištena za hemijsko vezivanje antibiotika ceftazidima. Vezivanje lijeka vršeno je iz vodenog rastvora antibiotika koncentracije c= 3,4´10–3 mol/L na sobnoj temperaturi (22±1 °C), a desorpcija lijeka u fiziološkom rastvoru. Količine vezanog i otpuštenog lijeka određene su spektrofotometrijski u UV području. Maksimalna količina vezanog lijeka (0,0243 mmol/g) dobijena je sorpcijom na modifikovani zavoj sa 2,276 mmol/g COOH, a maksimalna količina desorbovanog ceftazidima iznosila je 0,0238 mmol/g. Antimikrobno djelovanje uzoraka zavoja sa vezanim ceftazidimom testirano je in vitro na kulture Staphylococcus aureus, Bacillus subtilis i Escherichia coli metodom difuzije na agarnoj ploči. Najveća zona inhibicije dobijena je u odnosu na Escherichia coli. U radu se proučava uticaj hemijske strukture ceftazidima, pH vrijednosti rastvora iz koga se vrši sorpcija kao i trajanja sorpcije na količinu vezanog lijeka. Ustanovljeno je da se vezivanje lijeka ostvaruje jonskim i vodoničnim vezama lijeka sa oksidovanim celuloznim zavojem. Takođe, nađeno je da se količina ceftazidima koji se veže na vlakno iz vodenog rastvora antibiotika može povećati puferovanjem vodenog rastvora lijeka.
Downloads
Article Details
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:
- the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
- the right to produce prepublications, reprints, and special editions of the Manuscript;
- the right to translate the Manuscript into other languages;
- the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
- the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers;
- the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
- the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.
References
P. Škundrić, M. Kostić, A. Medović, J. Praskalo, Biološki aktivna vlakna na bazi modifikovanih polisaharida, Glasnik hem. i teh. RS 48 (2008) 93–103.
B. Rodić-Grabovac, R. Đuđić, Dobijanje oksidiranog celu-loznog vlakna sa anestezijskim i antimikrobnim djelo-vanjem, Glasnik hem. i teh. RS 47 (2008) 55–59.
H.O. Ammar, M. Ghorab, S.A. El-Nahhas, R. Kamel, Polymeric Matrix System for Prolonged Delivery of Tra-madol Hydrochloride, Part I: Physicochemical Eval-uation, AAPS Pharm. Sci. Tech. 10 (2009) 7–20.
P. Škundrić, A. Medović, M. Kostić, Fibrous systems with programmed biological activity and their application in medical practice, Autex. Res. J. 2 (2002) 78–84.
B. Rodić-Grabovac, R. Đuđić, N. Ilišković, Vezivanje anes¬tetika i antibiotika na karboksimetilcelulozu, Hem. Ind. 61 (2007) 203–207.
T.E. Nikiforova, V.A. Kozlov, Study of the Effect of Oxi-dative-Bisulfite Modification of the Cotton Cellulose on Its Ion Exchange Properties, Russ. J. Gen. Chem. 81 (2011) 2136–2141.
B. Rodić Grabovac, R. Đuđić, P. Sailović, The obtaining of materials with antibacterial activity by bonding of cefa¬zoline on modified cellulosic bandage, Contemp. Mater. V−2 (2014) 222–227.
A.C. Moffat, M.D. Osselton, B. Widdop, J. Watts, Clarkes Analysis of Drugs and Poisons in pharmaceuticals, body fluids and postmortem material, 4th ed., Pharmaceutical Press, London, 2011, pp. 1061.
V. Kumar, T. Yang, HNO3/H3PO4–NaNO2 mediated oxi-dation of cellulose – Preparation and characterization of bioabsorbable oxidized celluloses in high yields and with different levels of oxidation, Carbohydr. Polym. 48 (2002) 403–412.
USP (United States Pharmacopeia 23/National Formulary 18), 1995, Oxidized cellulose, p. 318.
R.L. Whistler, Methods in Carbohydrate Chemistry, Vol. III, Cellulose, Academic Press, New York and London, 1963, pp. 49–51.
J. H. Ortez, Disc diffusion testing, in: M.B. Coyle (Eds.), Manual of Antimicrobial Suspceptbility Testing, Amer-ican Society of Microbiology, Washington, 2005, pp. 39––52.
J. Buchenska, S. Slomkowski, J. Tazbir, E. Sobolewska, Antibacterial poly(ethylene .terephtalate) yarn contain-ing cephalosporin type antibiotic, Fibres. Text. East. Eur. 11 (2003) 41–47.
D. Klemm, B. Philipp, T. Heinze, U. Heinze, W. Wagen-knecht, Comprehensive Cellulose Chemistry, Vol. 1, Wiley-VCH, Weinheim, 1998, pp. 185–190.
A.H. Moreno, H.R.N. Salgado, Development and Vali-dation of the Quantitative Analysis of Ceftazidime in Powder for Injection by Infrared Spectroscopy, Phys. Chem. 2 (2012) 6–11.
M.A. Abounassif et al., Analytical Profiles of Drug Sub-stances, vol 19., Klaus Florey (Ed.), Academic press, San Diego, CA, 1992, pp. 100–106.
T. Kankkunen, Controlled transdermal drug delivery by iontophoresis and ion-exchange fiber, Academic dissert-ation, Department of Pharmacy, University of Helsinki, 2002, pp. 31
D. Cairns, Essentials of Pharmaceutical Chemistry, 4rd ed., Pharmaceutical Press, London, 2012, pp. 22.