CROSS-LINKED WHOLE CELLS FOR THE SUCROSE TRANSFRUCTOSYLATION REACTION IN A CONTINUOUS REACTOR Original scientific paper

Article Sidebar

PDF (705 kB)
Published: Dec 9, 2023
Updated: 09.12.2023
DOI: https://doi.org/10.2298/CICEQ221220015M
Keywords:
whole cells, geneous biocatalysts, packed bed reactors, fructooligosaccharides

Main Article Content

Beatriz Menossi Ribeiro
Institute of Science and Technology, Federal University of Alfenas (UNIFAL-MG), José Aurélio Vilela Road 11999, km 533, Poços de Caldas-MG, Zip Code 37715-400, Brazil
https://orcid.org/0000-0003-2387-7449
Leandro da Rin de Sandre Junior
Institute of Science and Technology, Federal University of Alfenas (UNIFAL-MG), José Aurélio Vilela Road 11999, km 533, Poços de Caldas-MG, Zip Code 37715-400, Brazil
Giancarlo de Souza Dias
Institute of Science and Technology, Federal University of Alfenas (UNIFAL-MG), José Aurélio Vilela Road 11999, km 533, Poços de Caldas-MG, Zip Code 37715-400, Brazil
Michelle da Cunha Abreu Xavier
2 Bioprocess Engineering and Biotechnology Department, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Sevilha, Gurupi-TO, Zip Code 77404-970, Brazil 3 Food Engineering Department, Federal University of Tocantins (UFT), NS Avenue 15, ALCNO-14, Palmas-TO, Zip Code 77001-090, Brazil
https://orcid.org/0000-0003-3564-6007
Alex Fernando de Almeida
3 Food Engineering Department, Federal University of Tocantins (UFT), NS Avenue 15, ALCNO-14, Palmas-TO, Zip Code 77001-090, Brazil
Elda Sabino da Silva
Industrial Biotechnology Laboratory, Institute for Technological Research (IPT-SP), Prof. Almeida Prado Avenue 532, University City, São Paulo-SP, Zip Code 05508-901, Brazil
https://orcid.org/0000-0001-8258-9936
Alfredo Eduardo Maiorano
Industrial Biotechnology Laboratory, Institute for Technological Research (IPT-SP), Prof. Almeida Prado Avenue 532, University City, São Paulo-SP, Zip Code 05508-901, Brazil
https://orcid.org/0000-0003-1230-5453
Rafael Firmani Perna
Institute of Science and Technology, Federal University of Alfenas (UNIFAL-MG), José Aurélio Vilela Road 11999, km 533, Poços de Caldas-MG, Zip Code 37715-400, Brazil
https://orcid.org/0000-0003-3195-8898
Sergio Andres Villalba Morales
Bioprocess Engineering and Biotechnology Department, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Sevilha, Gurupi-TO, Zip Code 77404-970, Brazil 3 Food Engineering Department, Federal University of Tocantins (UFT), NS Avenue 15, ALCNO-14, Palmas-TO, Zip Code 77001-090, Brazil

Abstract

Fructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT-301 cells used as a biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (AT) was presented by the biocatalyst prepared by cross-linking at 200 rpm and 45 min. The highest AT in the PBR was obtained at 50 °C, with flow rates from 3 mL min-1 to 5 mL min-1 and sucrose concentrations of 473 g L-1 and 500 g L-1. The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant AT of approximately 75 U g-1 and 300 U g-1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehyde-crosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors.


 

Article Details

How to Cite
Ribeiro, B. M. ., de Sandre Junior, L. da R., Dias, G. de S. ., Abreu Xavier, M. da C., de Almeida, A. F., Sabino da Silva, E. ., Maiorano, A. E., Perna, R. F., & Villalba Morales, S. A. . (2023). CROSS-LINKED WHOLE CELLS FOR THE SUCROSE TRANSFRUCTOSYLATION REACTION IN A CONTINUOUS REACTOR: Original scientific paper. Chemical Industry & Chemical Engineering Quarterly, 30(2), 99–110. https://doi.org/10.2298/CICEQ221220015M
Issue
Vol. 30 No. 2 (2024)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:

  • the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
  • the right to produce prepublications, reprints, and special editions of the Manuscript;
  • the right to translate the Manuscript into other languages;
  • the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
  • the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers; 
  • the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
  • the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.

Funding data

References

J.S. Cunha, C.A. Ottoni, S.A.V. Morales, E.S. Silva, A.E. Maiorano, R.F. Perna, Brazilian J. Chem. Eng. 36 (2019) 657—668. https://doi.org/10.1590/0104-6632.20190362s20180572.

M.B.P.O. Silva, D. Abdal, J.P.Z. Prado, G.S. Dias, S.A.V. Morales, M.C.A. Xavier, A.F. de Almeida, E.S. da Silva, A.E. Maiorano, R.F. Perna, Brazilian J. Food Technol. 24 (2021) 1—11. https://doi.org/10.1590/1981-6723.28320.

R.L. Garcia, G.S. Dias, S.A.V. Morales, M.C.A. Xavier, E.S. Silva, A.E. Maiorano, P.W. Tardioli, R.F. Perna, Brazilian J. Chem. Eng. 38 (2021) 273—285. https://doi.org/10.1007/s43153-021-00110-9.

C. Nobre, E.G.A. Filho, F.A.N. Fernandes, E.S. Brito, S. Rodrigues, J.A. Teixeira, L.R. Rodrigues, Lwt. 89 (2018) 58—64. https://doi.org/10.1016/j.lwt.2017.10.015.

G.S. Dias, E.D. Santos, M.C.A. Xavier, A.F. Almeida, E.S. Silva, A.E. Maiorano, R.F. Perna, S.A.V. Morales, J. Chem. Technol. Biotechnol. (2022). https://doi.org/10.1002/jctb.7163.

A.S.G. Lorenzoni, L.F. Aydos, M.P. Klein, M.A.Z. Ayub, R.C. Rodrigues, P.F. Hertz, J. Mol. Catal. B Enzym. 111 (2015) 51—55. https://doi.org/10.1016/j.molcatb.2014.11.002.

P. Zambelli, L. Tamborini, S. Cazzamalli, A. Pinto, S. Arioli, S. Balzaretti, F.J. Plou, L. Fernandez-Arrojo, F. Molinari, P. Conti, D. Romano, Food Chem. 190 (2016) 607—613. https://doi.org/10.1016/j.foodchem.2015.06.002.

K.H. Jung, S.H. Bang, T.K. Oh, H.J. Park, Biotechnol. Lett. 33 (2011) 1621—1624. https://doi.org/10.1007/s10529-011-0606-8.

E. Aguiar-Oliveira, F. Maugeri, Brazilian J. Chem. Eng. 28 (2011) 363—372. https://doi.org/10.1590/S0104-66322011000300002.

L.L. Faria, S.A.V. Morales, J.P.Z. Prado, G.S. Dias, A.F. de Almeida, M.C.A. Xavier, E.S. da Silva, A.E. Maiorano, R.F. Perna, Biotechnol. Lett. 43 (2021) 43—59. https://doi.org/10.1007/s10529-020-03016-7.

L.A. Garcia, J.P.Z. Prado, S.A.V. Morales, M.C.A. Xavier, M.S. Lopes, E.S. da Silva, A.E. Maiorano, R.F.K. Gunnewiek, R.F. Perna, Mater. Today Commun. 31 (2022). https://doi.org/10.1016/j.mtcomm.2022.103588.

L. Canilha, W. De Carvalho, Biotecnol. Ciência e Desenvolv. Ano IX. (2006) 48—57.

C.C. Castro, C. Nobre, M.E. Duprez, G. De Weireld, A.L. Hantson, Biochem. Eng. J. 118 (2017) 82—90. https://doi.org/10.1016/j.bej.2016.11.011.

R.C. Fernandez, C.A. Ottoni, E.S. Da Silva, R.M.S. Matsubara, J.M. Carter, L.R. Magossi, M.A.A. Wada, M.F. De Andrade Rodrigues, B.G. Maresma, A.E. Maiorano, Appl. Microbiol. Biotechnol. 75 (2007) 87—93. https://doi.org/10.1007/s00253-006-0803-x.

M.C.P. Gonçalves, S.A.V. Morales, E.S. Silva, A.E. Maiorano, R.F. Perna, T.G. Kieckbusch, J. Chem. Technol. Biotechnol. 95 (2020) 2473—2482. https://doi.org/10.1002/jctb.6429.

C.A. Ottoni, R. Cuervo-Fernández, R.M. Piccoli, R. Moreira, B. Guilarte-Maresma, E.S. Da Silva, M.F.A. Rodrigues, A.E. Maiorano, Brazilian J. Chem. Eng. 29 (2012) 49—59. https://doi.org/10.1590/S0104-66322012000100006.

M.A. Ganaie, H.K. Rawat, O.A. Wani, U.S. Gupta, N. Kango, Process Biochem. 49 (2014) 840—844. https://doi.org/10.1016/j.procbio.2014.01.026.

A.A. Homaei, R. Sahiri, F. Vianello, R. Stevanato, J. Chem. Biol. 6 (2013) 185—205. https://doi.org/10.1007/s12154-013-0102-9.

E.L. Cussler, Diffusion mass transfer in fluid systems, Cambridge University Press, New York (2009), p. 655. ISBN: 9780521871211.

H.S. Fogler, Elements of Chemical Reaction Engineering, Prentice Hall, (2019), p. 993. ISBN: 9780133887518.

C.J. Geankopolis, Transport Process and Unit Operations, Prentice-Hall International, New Jersey (1993), p. 937. ISBN: 0-13-045253-X.

M. Starzak, S.D. Peacock, Zuckerindustrie. 122 (1997) 380—387. ISSN 03448657. https://www.researchgate.net/publication/286862720.

M. Mathlouthi, P. Reiser, Sucrose: Properties and Applications, Chapman & Hall, (1995), p. 307. ISBN: 9781461361503.

I.M. Araújo, P.C. Becalette, E.S. da Silva, G.S. Dias, M.C.A. Xavier, A.F. de Almeida, A.E. Maiorano, S.A.V. Morales, R.F. Perna, J. Chem. Technol. Biotechnol. (2022). https://doi.org/10.1002/jctb.7255.

J. Nielsen, Adv. Biochem. Eng. Biotechnol. 46 (1992) 187—223. https://doi.org/10.1007/bfb0000711.

V.G. Elizei, S.M. Chalfoun, D.M. dos S. Botelho, P.P.R. Rebelles, Arq. Inst. Biol., Sao Paulo 81 (2014) 165—172. https://doi.org/10.1590/1808-1657001032012.

I.A. Soares, A.C. Flores, L. Zanettin, H.K. Pin, M.M. Mendonça, R.P. Barcelos, L.R. Trevisol, R.D. Carvalho, D. Schauren, C.L. de M.S.C. da Rocha, S. Baroni, Cienc. e Tecnol. Aliment. 30 (2010) 700—705. https://doi.org/10.1590/S0101-20612010000300021.

K. Long, H.M. Ghazali, A. Ariff, K. Ampon, C. Bucke, Biotechnol. Lett. 18 (1996) 1169—1174. https://doi.org/10.1007/bf01398317.

T. Sun, W. Du, D. Liu, L. Dai, Process Biochem. 45 (2010) 1192—1195. https://doi.org/10.1016/j.procbio.2010.03.037.

O. Barbosa, R. Torres, C. Ortiz, R. Fernandez-Lafuente, Process Biochem. 47 (2012) 1220—1227. https://doi.org/10.1016/j.procbio.2012.04.019.

J.M. Guisan, Immobilization of Enzymes and Cells, Humana Press, New York (2013), p. 375. ISBN: 978-1-62703-550-7.

A.E. Maiorano, E.S. da Silva, R.F. Perna, C.A. Ottoni, R.A.M. Piccoli, R.C. Fernandez, B.G. Maresma, M.F.A. Rodrigues, Biotechnol. Lett. 42 (2020) 2619—2629. https://doi.org/10.1007/s10529-020-03006-9.

J.S. Lim, J.H. Lee, J.M. Kim, S.W. Park, S.W. Kim, Biotechnol. Bioprocess Eng. 11 (2006) 100—104. https://doi.org/10.1007/BF02931891.

J. Su, S. Jia, X. Chen, H. Yu, J. Appl. Phycol. 20 (2008) 213—217. https://doi.org/10.1007/s10811-007-9221-4.

I. Mahasiswa, E. Indonesia, U.G. Mada, World J. Chem. 4 (2009) 34—38.

Y. Bakri, A. Mekaeel, A. Koreih, Brazilian Arch. Biol. Technol. 54 (2011) 659—664. https://doi.org/10.1590/s1516-89132011000400003.

I.L. Furlani, B.S. Amaral, R. V. Oliveira, Q.B. Cassa, Quim. Nova. 43 (2020) 463—473. https://doi.org/10.21577/0100-4042.20170525.

P.A. Fields, Comp. Biochem. Physiol. - A Mol. Integr. Physiol. 129 (2001) 417—431. https://doi.org/10.1016/S1095-6433(00)00359-7.

V.M. Almeida, S.R. Marana, PLoS One. 14 (2019) 1—8. https://doi.org/10.1371/journal.pone.0212977.

E. Gomes, M.A.U. Guez, N. Martin, R. da Silva, Quim. Nova. 30 (2007) 136—145. https://doi.org/10.1590/S0100-40422007000100025.

D. de Andrades, N.G. Graebin, M.K. Kadowaki, M.A.Z. Ayub, R. Fernandez-Lafuente, R.C. Rodrigues, Int. J. Biol. Macromol. 129 (2019) 672—678.https://doi.org/10.1016/j.ijbiomac.2019.02.057.

M. Antošová, M. Polakovič, Chem. Pap. 55 (2001) 350—358. https://www.chempap.org/file_access.php?file=556a350.pdf.

P. Monsan, J. Mol. Catal. 3 (1978) 371—384. https://doi.org/10.1016/0304-5102(78)80026-1.

R. Satar, M.A. Jafri, M. Rasool, S.A. Ansari, Brazilian Arch. Biol. Technol. 60 (2017) 1—12. https://doi.org/10.1590/1678-4324-2017160311.

H. Veny, M.K. Aroua, N.M.N. Sulaiman, Chem. Eng. J. 237 (2014) 123—130. https://doi.org/10.1016/j.cej.2013.10.010.

W. Sieng, A. Yuniarto, J. Environ. Chem. Eng. 7 (2019) 103185. https://doi.org/10.1016/j.jece.2019.103185.

Most read articles by the same author(s)