Physico-chemical and biological properties of dental calcium silicate cements - literature review

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Vol 73 No 5
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Published: Nov 14, 2019
DOI: https://doi.org/10.2298/HEMIND190614027I
Keywords:
Bioactive material, dentine substituent, portland cement, mineral trioxide aggregate, BiodentineTR

Main Article Content

Dragan V. Ilić
Department for Conservative Dentistry, School of Dental Medicine, University of Belgrade, Serbia
Đorđe M. Antonijević
School of Dental Medicine, University of Belgrade, Belgrade, Serbia; Laboratory of Atomic Physics, Institute for Nuclear Sciences "Vinča", University of Belgrade, Belgrade and Laboratory of Anthropology, School of Medicine, University of Belgrade, Belgrade,
Vladimir M. Biočanin
Faculty of Dentistry, University of Business Academy, Novi Sad
Božana Čolović
Laboratory for atomic physics, Institute for nuclear sciences "Vinca", University of Belgrade, Serbia
Vesna Danilović
School of Dental Medicine, University of Belgrade, Belgrade
Vladimir S. Komlev
A. A. Baikov Institute of Metallurgy and Materials Science (A.A. Baikov IMET RAS), Moscow
Anastasia Y. Teterina
A. A. Baikov Institute of Metallurgy and Materials Science (A.A. Baikov IMET RAS), Moscow
Vukoman R. Jokanović
Laboratory of Atomic Physics, Institute for Nuclear Sciences "Vinča", University of Belgrade, Belgrade and ALBOS d.o.o

Abstract

Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.

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How to Cite
Ilić, D. V., Antonijević, Đorđe M., Biočanin, V. M., Čolović, B., Danilović, V., Komlev, V. S., Teterina, A. Y., & Jokanović, V. R. (2019). Physico-chemical and biological properties of dental calcium silicate cements - literature review. HEMIJSKA INDUSTRIJA (Chemical Industry), 73(5), 281–294. https://doi.org/10.2298/HEMIND190614027I
Issue
Vol. 73 No. 5 (2019)
Section
Engineering of Materials - Biomaterials
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Author Biographies

Dragan V. Ilić, Department for Conservative Dentistry, School of Dental Medicine, University of Belgrade, Serbia

PhD

Đorđe M. Antonijević, School of Dental Medicine, University of Belgrade, Belgrade, Serbia; Laboratory of Atomic Physics, Institute for Nuclear Sciences "Vinča", University of Belgrade, Belgrade and Laboratory of Anthropology, School of Medicine, University of Belgrade, Belgrade,

PhD

Vladimir M. Biočanin, Faculty of Dentistry, University of Business Academy, Novi Sad

Associate prof PhD, Dpt. for oral Surgery

Božana Čolović, Laboratory for atomic physics, Institute for nuclear sciences "Vinca", University of Belgrade, Serbia

PhD

Vladimir S. Komlev, A. A. Baikov Institute of Metallurgy and Materials Science (A.A. Baikov IMET RAS), Moscow

PhD vice director

Anastasia Y. Teterina, A. A. Baikov Institute of Metallurgy and Materials Science (A.A. Baikov IMET RAS), Moscow

PhD

Vukoman R. Jokanović, Laboratory of Atomic Physics, Institute for Nuclear Sciences "Vinča", University of Belgrade, Belgrade and ALBOS d.o.o

PhD

References

Hench LL, Polak JM. Third-generation biomedical materials. Science. 2002; 8:1014-1017.

Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review. Part I: chemical, physical and antibacterial properties. J Endod. 2010; 36:16-27.

Dammaschke T. The history of direct pulp capping. J Hist Dent. 2008; 56:9-23.

Schröder U. Effects of calcium hydroxide pulp-capping agents on pulp cell migration, proliferation, and differentiation. J Dent Res.1985; 64:541-8.

Rabchinsky J, Donly KJ. A comparison of glass-ionomer cement and calcium hydroxide liners in amalgam restorations. Int J Periodontics Restorative Dent. 1993; 13:378-83.

Saad AY. Calcium hydroxide and apexogenesis. Oral Surg Oral Med Oral Path. 1988; 66:499-501.

Watson TF, Atmeh AR, Sajini S, Cook RJ, Festy F. Present and future of glass-ionomers and calcium-silicate cements as bioactive materials in dentistry: Biophotonics-based interfacial analyses in health and disease. Dent Mater. 2014; 30:50–61.

https://www.septodontcorp.com/technology-and-products/endodontics-and-restorative/biodentine/, accessed November 2019.

Torabinejad M, Watson TF, Pitt Ford TR. Sealing ability of a mineral trioxide aggregate when used as a root end filling material. J Endod. 1993; 19:591-595.

Schembri M, Peplow G, Camilleri J. Analises of heavy metals in mineral trioxide aggregate and Portland cement. J Endod. 2010; 36:1210-15.

Roberts HW, Toth JM, Berzins DW, Charlton DG. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dent Mater. 2008; 24:149-64.

Camilleri J. Analyses of heavy metals in mineral trioxide aggregate and Portland cement. Int Endod J. 2007; 40:462-70.

Asgary S, Parirokh M, Eghbal MJ, Brink F. Chemical differences between white and gray mineral trioxide aggregate. J Endod. 2005; 31:101–3.

Jafari F, Jafari S. Composition and physicochemical properties of calcium silicate based sealers: A review article. J Clin Exp Dent. 2017; 9:e1249-e1255.

Camilleri J. Mineral trioxide aggregate in dentistry. From preparation to application. E book, Springer, 2014, ISBN: 3642551564. Chapter: Composition and setting reaction. J. in ebook Verlag, Berlin-Heidelberg. 19-37.

Parirokh M, Torabinejad M. 10 calcium silicate-based cements. Pocket Dentistry. Available at: http://pocketdentistry.com/10 calcium-silicate-based-cements. 2017.

Singh H, Kaur M, Markan S, Kapoor P. Biodentine: A Promising Dentin substitute. J Interdiscipl Med Dent Sci. 2014; 2: 140. doi: 10.4172/ 2376-032X.1000140

Nilsen BW, Jensen E, Ortengren U, Michelsen VB. Analysis of organic components in resin modified pulp capping materials: critical considerations. Europ Jour Oral Sci. 2017; 125:183-194.

Nagas E, Uyanik MO, Eymirli A, Cehreli ZC, Vallittu PK, Lassila LV, Durmaz V. Dentin moisture conditions affect the adhesion of root canal sealers. J Endod. 2012; 38:240-44.

Asgary S, Shahabi S, Jafarzadeh T, Amini S, Kheirieh S. The properties of a new endodontic material. J Endod. 2008; 34:990-993.

Utneja S, Roongta R, Talwar NS, Verma M. Current perspectives of bio-ceramic technology in endodontics: calcium enriched mixture cement - review. Restor Dent Endo. 2015; 40:1-13.

Cetenovic B, Prokic B, Vasilijic S, Dojcinovic B, Magic M, Jokanovic V, Markovic D. Biocompatibility investigation of new endodontic materials based on nanosynthesized calcium silicates combined with different radiopacifiers. J Endod. 2017; 43:425-432.

Lemos Martins SS, Gabardo MC, Castiglia GC, Dias MN, Baratto-FF, Correr NGM, Leonardi DP. Bond strength of self-adhesive resin cement to different root perforation materials. J Endod. 2016; 42:1819-21.

Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod. 1995; 21:349–53.

Matovic I, Ilic VD, Petrovic R, Ostojic D. The application of MTA as apical plug for root canal obturation (in vitro study). Serb Dent J. 2018; 65:1-5.

Aqrabawi J. Sealing ability of amalgam, super EBA cement and MTA when used as retrograde filling materials. Br Dent J. 2000; 188:266-68.

Von Arx T. Apical surgery: A review of current techniques and outcome. Saudi Dent J. 2011; 23:9–15.

Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review-part II: leakage and biocompatibility investigations. J Endod. 2010; 36:190-202.

Guimares BM, Vivan RR, Piazza B, Alcalde MP, Bramante CM, Duarte MH. Chemical-physical properties and apatite-forming ability of mineral trioxide aggregate flow. J Endod. 2017; 43:1692-96.

MalkonduÖ, KarapinarKazandağ M, Kazazoğlu E. A review on Biodentine, a contemporary dentine replacement and repair material. BioMed Research International. 2014, 2014:160951.

Akbulut MB, Arpaci PU, Eldeniz AU. Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts. Restor Dent Endod. 2018; 43:e24.

Solanki NP, Venkappa KK, Shah NC. Biocompatibility and sealing ability of mineral trioxide aggregate and biodentine as root-end filling material: A systematic review. J Conserv Dent. 2018; 21:10-15.

Shetty S, Hiremath G, Yeli M. A comparative evaluation of sealing ability of four root end filling materials using fluid filtration method: An in vitro study. J Conserv Dent. 2017; 20:307-310.

Benoist FL, Ndiaye FG, Kane AW, Benoist HM, Farge P. Evaluation of mineral trioxide aggregate-MTA versus calcium hydroxide cement Dycal in the formation of a dentine bridge: a randomized controlled trial. Int Dent J. 2012; 62: 33-39.

Camilleri J. Hydration characteristics of Biodentine and Theracal used as pulp capping materials. Dent Mater. 2014; 30:709-15.

Maheaswari R, Jeeva RM. Biodentine: Periodontal perspective. Journ App Dent Sci. 2017; 3:12-16.

Harpreet S, Mandeep K, Sheenam M, Pooja K. Biodentine a promising dentin substitute. J Interdiscipl Med Dent Sci. 2014; 2:140.

Hashem AA, Wanees Amin SA. Effect of acidity on dislodgment resistance of mineral trioxide aggregate and Bioaggregate in furcation perforations: in vitro comparative study. J Endod. 2012; 38:245-49.

Biocanin V, Antonijevic Đ, Postic S, Ilic VD, Vukovic Z, MilicM, Fan Y, Li Z, Brković B, Đurić M. Marginal gaps between 2 calcium silicate and glass ionomer cements and apical root dentin. J Endod. 2018; 44:816-821.

Zarra T, Lambrianidis T, Vasiliadis L, Gogos C. Effect of curing conditions on physical and chemical properties of MTA. Int Endod J. 2018; 51:1279-1291.

De Deus G, Canabarro A, Alves G, Linhares A, Senne MI, Granjeiro, JM. Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells. J Endod. 2009; 35:1387-90.

Guven Y, Tuna EB, Dincol ME, Ozel E, Yilmaz B, Aktoren O. Long-Term fracture resistance of simulated immature teeth filled with various calcium silicate-based materials. Biomed Res Int. 2016; 2863817.

Sagsen B, Ustün Y, Demirbuga S, Pala K. Push-out bond strength of two new calcium silicate-based endodontic sealers to root canal dentine. Int Endod J. 2011; 44:1088-91.

Darag AM, Fayyad DM. Adhesives in endodontics. Part II: Role of adhesion in root canal obturation. Endodontic Practice Today.2011;5:87-105.

Soheilipour E, Kheirieh S, Madani M, Baghban AA, Asgary S. Particle size of a new endodontic cement compared to Root MTA and calcium hydroxide. Iran Endod J, 2009; 4:112–16.

Sahebi S, Nabavizadeh M, Dolatkhah V, Jamshidi D. Short term effect of calcium hydroxide, mineral trioxide aggregate and calcium-enriched mixture cement on the strength of bovine root dentin. Iran Endod J. 2012; 7:68–73.

Oskoee SS, Kimyai S, Bahari M, Motahari P, Eghbal MJ, Asgary S. Comparison of shear bond strength of calcium-enriched mixture cement and mineral trioxide aggregate to composite resin. J Contemp Dent Pract. 2011; 12: 457-62.

Milani AS, Rahimi S, Borna Z, Jafarabadi MA, Bahari M, Deljavan AS. Fracture resistance of immature teeth filled with mineral trioxide aggregate or calcium-enriched mixture cement: An ex vivo study. Dental Research Journ (Isfahan). 2012; 9:299-304.

Vivan RR, Zapata RO, Zeferino MA, Bramante CM, Bernardineli N, Garcia RB Hungaro Duarte MA, Tanomaru Filho M, Gomes de Moraes I. Evaluation of the physical and chemical properties of two commercial and three experimental root-end filling materials. Oral Surg Oral Med Oral Pathol Oral RadiolEndod.2010; 110:250-56.

Guneser MB, Akbulut AU, Eldeniz MB. Effect of various endodontic irrigants on the push-out bond strength of Biodentine and conventional root perforation repair materials. J Endod.2013; 39:380-384.

Belio-Reyes IA, Bucio L, Cruz Chavez E. Phase composition of ProRoot mineral trioxide aggregate by x ray powder diffraction. J Endod. 2009; 35:875-878.

Kuga MC, Duarte MA, Sant'anna-Júnior A, Keine KC, Faria G, Dantas AA, GuiottFA. Effects of calcium hydroxide addition on the physical and chemical properties of a calcium silicate-based sealer. J Appl Oral Sci. 2014; 22:180–184.

Alsubait SA, Hashem Q, Al Hargan N, Al Mohameed K, Alkhatani A. Comparative evaluation of push-out bond strength of ProRoot MTA, bioaggregate and biodentine. JournContemp Dent Pract. 2014; 1:336-40.

Abraham SB, Gaintantzopoulou MD, Eliades G. Cavity Adaptation of Water-Based Restoratives Placed as Liners under a Resin Composite. Int J Dent. 2017; 2017:5957107.

Jain A, Bhadoria K, Hada HS. Spectrophotometric evaluation of calcium ion release from different calcium hydroxide preparations: an in vitro study. J Oral Res. 2017; 6:61-63.

Schwendicke F, Al-Abdi A, Pascual Moscardó A, Ferrando Cascales A, Sauro S. Remineralization effects of conventional and experimental ion-releasing materials in chemically or bacterially-induced dentin caries lesions. Dent Mater. 2019; 35(5):772-779.

Qi YP, Li N, Niu LN, Primus CM, Ling JQ, Pashley DH, Tay FR. Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate. Acta Biomater. 2012; 8: 836-42.

Gurcan AT, Seymen F. Clinical and radiographic evaluation of indirect pulp capping with three different materials: a 2-year follow-up study. Eur J Paediatr Dent. 2019; 20:105-110.

Gandolfi MG, Siboni F, Prati C. Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. Int Endod J. 2012; 45:571-9.

Borges RP, Sousa-Neto MD, Versiani MA, Rached-Júnior FA, De-Deus G, Miranda CE, Pecora JD. Changes in the surface of four calcium silicate-containing endodontic materials and an epoxy resin-based sealer after a solubility test. Int Endod J.2012; 45:419-28.

Alzraikat H, Taha NA, Hassouneh H. Dissolution of a mineral trioxide aggregate sealer in endodontic solvents compared to conventional sealers. Braz Oral Res. 2016; 30.

Kamal EM, Nabih SM, Obeid RF, Abdelhameed MA. The reparative capacity of different bioactive dental materials for direct pulp capping. Dent Med Probl. 2018; 55:147-152.

Antonijevic D, Jeschke A, Colovic B, Milovanovic P, Jevremovic D, Kisic D. vomScheidt A, Hahn M, Amling M, Jokanovic V, Busse B, Djuric M. Addition of a fluoride-containing radiopacifier improves micromechanical and biological characteristics of modified calcium silicate cements. J Endod. 2015; 41:2050-57.

Antonijevic D, Milovanovic P, Riedel C, Hahn M, Amling M, Busse B, Djuric M. Application of reference point indentation for micro-mechanical surface characterization of calcium silicate based dental materials. Biomedical Microdevices. 2016; 18:12-17.

Antonijević D, Ilić D, Medić V, Dodić S, Obradović-Djuriĉić K, Rakoĉević Z. Evaluation of conventional and digital radiography capacities for distinguishing dental materials on radiograms depending on the present radiopacifying agent. Vojnosanit Pregl. 2014; 71:1006-12.

Antonijevic D, Medigovic I, Zrilic M, Jokic B, Vukovic Z, Todorovic L. The influence of different radiopacifying agents on the radiopacity, compressive strength, setting time, and porosity of Portland cement. Clin Oral Investig. 2014; 18:1597-604.

Mozynska J, Metlerski M, Lipski M, Nowicka A. Tooth discoloration induced by different calcium silicate cements: a systemic review of in vitro studies. J Endod. 2017; 43: 1593-1601.

Best SM, Porter AE, Thian ES, Huang J. Bioceramics: past, present and future. J Eur Ceram Soc. 2008; 28:1319-27.

Cancedda R, Dozin B, Giannoni P, Quarto R. Tissue engineering and cell therapy of cartilage and bone. Matrix Biol. 2003; 22:81-91.

Williams D. Benefit and risk in tissue engineering. Materials Today. 2004; 7:24–29.

Hwang YC, Lee SH, Hwang IN, Kang IC, Kim MS, Kim SH, Son HH, Oh WM. Chemical composition, radiopacity, and biocompatibility of Portland cement with bismuth oxide. Oral Surg Oral Med Oral Pathol Oral RadiolEndod. 2009; 107: e96-102.

Grazziotin SR, Nekoofar MH, Davies TE, Bafail A, Alhaddar E, Hubler R, Busato AL, Dummer PM. Effect of bismuth oxide on white MTA: chemical characterization and physical properties. Int Endod J. 2014; 47:520-33.

Bosso-Martelo R, Guerreiro-Tanomaru JM, Viapiana R, Berbert FL, Basso BMI, Tanomaru-Filho M. Calcium silicate based cements associated with micro and nanoparticle radiopacifiers: physicochemical properties and bioactivity. Int Schol Res Notices. 2015; 23:874283.

Laurent P, Camps J, About I. Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J.2012; 45:439-48.

Zhaofei L, Cao L, Fan M, Xu Q. Direct pulp capping with calcium hydroxide or Mineral Trioxide Aggregate: A Meta-analysis. J Endod. 2015; 41:1412-7.

Yuan Z, Peng B, Jiang H, Bian Z, Yan P.J Effect of bioaggregate on mineral-associated gene expression in osteoblast cells. J Endod. 2010; 36:1145-8.

De-Deus G, Canabarro A, Alves GG, Marins JR, Linhares AB, Granjeiro JM. Cytocompatibility of the ready-to-use bioceramic putty repair cement iRoot BP Plus with primary human osteoblasts. Int Endod J. 2012; 45:508-13.

Zhang W, Li Z, Peng B. Ex vivo cytotoxicity of a new calcium silicate–based canal filling material. Int Endod J.2010; 43: 769–74.

Shamkhalov GS. Comparative study of antimicrobial activity of "Biodentin" and "Rootdent" cements and "Futurabond NR" adhesive. Stomatologiia (Mosk). 2013; 92:37-9.

Petrovic V, Opacic Galic V, Jokanovic V, Jovanovic M, Basta Jovanovic G, Zivkovic S. Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissues of rats. Acta Vet. 2012; 62:697-708.

Opacic-Galic V, Petrovic V, Jokanovic V, Zivkovic S. Histological evaluation of tissue reactions to newly synthetized calcium silicate- and hydroxyapatite-based bioactive materials: in vivo study. SrpArhCelokLek. 2017; 145:370-7.

Paraš S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. Int Endod J. 2019 Feb 25.

Torabinejad M, Hong CU, Pitt Ford TR, Kaiyawasam SP. Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod. 1995; 21:569-71.

Popovic Bajic M, Petrovic V, Opacic Galic V, Danilovic V, Jokanovic V, Prokic B, Prokić BB, Živković S. Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite. Serb Dent J. 2016; 63:83-92.

Popovic Bajic M, Prokic BB, Prokic B, Jokanovic V, Danilovic V, Zivkovic S. Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine on pulp tissue. Acta Vet. 2013; 63: 347-60.

Ćetenović B, Čolović B, Vasilijić S, Prokić B, Pašalić S, Jokanović V, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agents-biocompatibility study. J Mater Sci Mater Med. 2018; 29:190.

Cetenovic B, Prokic B, Vasilijic S, Dojcinovic B, Magic M, Jokanovic V, Markovic D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. J Endod. 2017; 43:425-432.

Alex G. Direct and indirect pulp capping: A brief history, material innovations and clinical case report. Compend Contin Educ Dent. 2018; 39:182-89.

Juneja P, Kulkarni S. Clinical and radiographic comparison of biodentine, mineral trioxide aggregate and formocresol as pulpotomy agents in primary molars. Eur Arch Paediatr Dent. 2017; 18:271-78.

Dexton AJ, Vasundara Y. Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics. J Conservat Dent. 2014; 17: 487–90.

Torabinejad M, Parirokh M, Dummer PMH. Mineral trioxide aggregate and other bioactive endodontic cements: An updated overview - Part II: Other clinical applications and complications. Int Endod J. 2018; 51:284-317.

Kundzina R, Stangvaltaite L, Eriksen HM, Kerosuo E. Capping carious exposures in adults: a randomized controlled trial investigating mineral trioxide aggregate versus calcium hydroxide. Int Endod J. 2017; 50:924-32.

Mohammadi Z, Dummer P. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J. 2011; 44:697-730.

Caliskan MK, Tekin U, Kaval ME, Solmaz MC. The outcome of apical microsurgery using MTA as the root-end filling material: 2 6 year follow-up study. Int Endod J. 2016; 49:245-254.

Zeyneb AA, Al-DahanAbeer M, Zwain Aseel Haidar H M, Al-Assadi. Clinical and radiographical evaluation of pulpotomy in primary molars treated with Pulpotec (PD), Formocresol and mineral trioxide aggregate (MTA). Journ Baghdad College Dentistry. 2013; 25: 164-170.

Bakhtiar H, Nekoofar MH, Aminishakib P, Abedi F, Naghi MoosaviF, Esnaashari E, Azizi A, Esmailian S, Ellini MR, Mesgarzadeh V, Sezavar M, About I. Human pulp responses to partial pulpotomy treatment with TheraCal as compared with Biodentine and ProRootMTA: A clinical trial. J Endod. 2017; 43:1786-91.

Brizuela C, Ormeño A, Cabrera C, Cabezas R, Silva CI, Ramírez V, Mercade M. Direct pulp capping with calcium hydroxide, Mineral Trioxide Aggregate and Biodentine in permanent young teeth with caries: A randomized clinical trial. J Endod. 2017; 43:1776-80.

Petrou MA, Alhamoui FA, Welk A, Altarabulsi MB, Alkilzy M, Splieth C. A randomized clinical trial on the use of medical Portland cement, MTA and calcium hydroxide in indirect pulp treatment. Clin Oral Investig. 2014; 18:1383-89.

Menon NP, Varma BR, Janardhanan S, Kumaran P, Xavier AM, Govinda BS. Clinical and radiographic comparison of indirect pulp treatment using light-cured calcium silicate and mineral trioxide aggregate in primary molars: A randomized clinical trial. Contemp Clin Dent. 2016; 7:475-80.

Fallahinejad MG, Asgharian TJ, Iri S, Asgary S. Direct pup capping with calcium enriched mixture in primary molar teeth: a randomized clinical trial. Iran Endod J. 2010; 5:27-30.

Fallahinejad MG, Asqharian JT, Iri S, Asgary S. Treatment outcomes of primary molars direct pulp capping after 20 months: a randomized controlled trial. Iran Endod J. 2013; 8:149-52.

Schwendicke F, Stolpe M. Direct pulp capping after a carious exposure versus root canal treatment: a cost-effectiveness analysis. J Endod. 2014; 40:1764-70.

Kato C, Suzuki M, Shinkai K, Katoh Y. Histopathological and immunohistochemical study on the effects of a direct pulp capping experimentally developed adhesive resin system containing reparative dentin-promoting agents. Dent Mater J. 2011; 30:583-97.

Marques MS, Wesselink PR, Shemesh H. Outcome of direct pulp capping with mineral trioxide aggregate: a prospective study. J Endod. 2015; 41:1026-31.

Villat C, Grosgogeat B, Seux D, Farge P. Conservative approach of a symptomatic carious immature permanent tooth using a tricalcium silicate cement (Biodentine): A case report. Restor Dent Endod. 2013; 38:258-262.

Asgary S, Shirvani A, Fazlyab M. MTA and ferric sulfate in pulpotomy outcomes of primary molars: a systematic review and meta-analysis. J Clin Pediatr Dent. 2014; 39:1-8.

Martens L, Rajasekharan S, Cauwels R. Pulp management after traumatic injuries with a tricalcium silicate-based cement (Biodentine™): a report of two cases up to 48 months follow-up. Eur Arch Paediatr Dent. 2015; 16:491-496.

Soni HK. Biodentine pulpotomy in mature permanent molar: A case report. J Clin Diagn Res. 2016; 10: ZD09-11.

Cetenovic B, Markovic D, Petrovic B, Peric T, Jokanovic V. Use of mineral trioxide aggregate in the treatment of traumatized teeth in children - two case reports. Vojnosanit Pregl. 2013; 70:781-784.